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KMID : 0604520050310010013
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Journal of the Society of Cosmetic Scientists of Korea
2005 Volume.31 No. 1 p.13 ~ p.16
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Developing a Protein-chip for Depigmenting Agents Screening
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Kim Eun-Ki
Kwak Eun-Young
Han Jeong-Seon
Lee Hyang-Bok
Shin Jeong-Hyun
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Abstract
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For the high-throughput-screening system (HTS) of depigmenting agents using a protein chip, effects of oligonucleotide-inhibitor sequence on the binding of Mitf protein to E box of MC1R was investigated. The sequence of oligonucletide-inhibitor affected the binding of the target DNA to Mitf, depending on the location of the sequence variation in the inhibitor nucleotide. The oligonucletide-inhibitor that changed the CATGTG sequence didn’’t show enough inhibition of the target DNA to Mitf, whereas significant inhibition was observed when the sequence outside the CATGTG was changed. This result indicated that CATCTG is crucial sequence for the binding of Mitf to I-box which initiates the transcription of pigmenting genes.
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KEYWORD
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Mitf(microrhthalmia transcription factor), E-box, microarray
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